Firstly
you have to be interested in finding out if adaptive evolution has occured
somwhere in a dataset
The sequences must be
protein coding nucioltide sequences
This dataset must
aligned and homologous.
An appropriate outgroup
needs to be included.
The dataset must be FASTA
format.
There is no limit to the number
or size of the sequences that can be entered into the program. (You are only
restricted by the ammount of memory that your system can allocate to the program).